Safranin O is the cationic dye that finds usage in medical field for staining acidic proteoglycan that are present in cartilage tissues and work as indicator cell chondrogenesis. The Safranin O stain is available in powder form which makes it easily dissolved in de-ionized water for making staining solution. When bound to glycosaminoglycan, Safranin O shows orange-red color.

Diethyl Safranine finds application in cytology and histology fields where it is used as counter stain in some of the staining procedures including coloring of all cell nuclei in red color. Further, it is also suitable for detection of mucin, mast cell granules and cartilage.

Biological Stains are dyes which are frequently used in biology and medicine to highlight structures in biological tissues. These biological stains are also used in the identification and study of polymer structures.